Often known as the “Australia Antigen assay,” the Hepatitis B Surface Antigen (HBsAg) test is a vital diagnostic technique that determines whether the hepatitis B virus (HBV) is present in the blood. The first sign of acute hepatitis B is HBsAg, which is also the main marker for determining whether an HBV infection is active. This test is crucial for preventing the spread of hepatitis B since the presence of HBsAg in the blood indicates that the subject is contagious and capable of infecting others.The Australia Antigen assay’s description, which includes the term “health heart diagnostics” (HHD), highlights the assay’s significance not only for liver health but also for cardiovascular health. Cirrhosis and other severe liver diseases can result from chronic hepatitis B.